Plasmid

Part:BBa_K5181018

Designed by: Eeshani Umesh Abhyankar   Group: iGEM24_IISER-TVM   (2024-09-24)


pRGPDuo_SaSSY_FPPS

This part contains two enzymes, SaSSY and FPPS, under two different promoters.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1760
    Illegal PstI site found at 13
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1760
    Illegal PstI site found at 13
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1760
    Illegal BglII site found at 1366
    Illegal BglII site found at 4118
    Illegal XhoI site found at 3070
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1760
    Illegal PstI site found at 13
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1760
    Illegal PstI site found at 13
    Illegal NgoMIV site found at 7942
    Illegal AgeI site found at 5373
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1959
    Illegal BsaI.rc site found at 1478
    Illegal SapI site found at 4319
    Illegal SapI.rc site found at 191
    Illegal SapI.rc site found at 5390


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Categories
Parameters
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